Research

Electron Microscopy
SEM 1 (60 KB) - Transverse section at the level of the optic placode.
SEM 2 (60 KB) - Sagittal section of stage 10 embryo.
SEM 3 (92 KB) - Transverse section of embryo at spinal cord level.
TEM 1 (144 KB) - Section through luminal side of neural tube.

In Situ Hybridization
In Situ 1 (120 KB) - FGF-19 and Wnt-8c.
In Situ 2 (156 KB) - FGF-19 in stage 8 embryo.
In Situ 3 (184 KB) - α6integrin in stage 4 embryo.

Immunohistochemistry
Immunohistochemistry 1 (180 KB) - Lim1/2 antibody developed with DAB, Fz-4 in situ hybridization with DIG labeled probe.
Immunohistochemistry 2 (228 KB) - Section from Immunohistochemistry 1.
Immunohistochemistry 3 (140 KB) - Engrailed-2 in stage 10 embryo.

Grafting
Grafting 1 (200 KB) - Grafting with fluorescent labeled cells.
Grafting 2 (76 KB) - Grafting with fluorescently labeled quail cells.
Grafting 3 (144 KB) - Steps in making a chimera.
Grafting 4 (100 KB) - Section of neural tube from Grafting 2 after further incubation showing QCPN positive quail cells.

EC Culture
EC Culture 1 (128 KB) - Embryo on filter paper, cultured on agar/albumen dish.
EC Culture 2 (220 KB) - Stills of EC culture embryo photographed using timelapse videomicroscopy.

Collagen Culture
Collagen Culture 1 (208 KB) - Bead and cell implantation in explanted tissue to examine levels of FGF-19 and Wnt-8c in inner ear induction.
Collagen Culture 2 (140 KB) - Explanted tissue with beads soaked in FGF-19 and probed for Dlx-5 transcripts, cultured in collagen.

Microsurgery
Microsurgery 1 (120 KB) - Embryo cultured on agar/albumen substrate. Whole streak removed.
Microsurgery 2 (148 KB) - Explant removed from an embryo.
Microsurgery 3 (152 KB) - A small wedge of tissue removed from the ectoderm at the margin of the area pellucida.

Electroporation
Electroporation 1 (240 KB) - GFP plasmid electroporated into embryos in EC culture.
Electroporation 2 (160 KB) - Easy does it!

Cell Tracking
Cell Tracking 1 (76 KB) - Wholemount embryo with fluorescently labeled cells at 0 hours.
Cell Tracking 2 (188 KB) - Arrows show location of fluorescently labeled cells at 24 hours from Cell Tracking 1.
Cell Tracking 3 (132 KB) - Embryo injected with DiI/CRSE mixture at 0 hours.
Cell Tracking 4 (128 KB) - Embryo injected with DiI/CRSE mixture after 12 hours from Cell Tracking 3.
Cell Tracking 5 (148 KB) - Embryo grafted with a fluoresently labeled Hensen's node and images captured by timelapse video microscopy.
Cell Tracking 6 (184 KB) - Embryo double injected with DiO/CFSE and DiI/CRSE at 0 hours.
Cell Tracking 7 (200 KB) - Embryo double injected with DiO/CFSE and DiI/CRSE at 12 hours.

Chick Expressions Website

Susan Chapman has designed and maintains the Chick Expressions Website.  It features various genes at different stages of chick development. All images on the site are the property of Susan Chapman and should not be reproduced without her prior written permission. To get Susan's contact information please visit the people page.